Shirley, NY -- (SBWIRE) -- 09/30/2015 -- Creative Biolabs is glad to announce a new series of stable cell lines services.
Creating stable cell lines is a key method in reducing the production costs of biopharmaceuticals. Creative Biolabs uses a modified FRT/Flp-In recombinase system to produce human proteins in CHO cells and 293 cells for stable expression. It has pre-selected CHO cell lines and 293 cell lines that contain a single stably integrated FRT site at a transcriptionally active genomic locus, and these cell lines are proved to have the ability to produce high level expressers of recombinant proteins.
Creative Biolabs has also developed a unique series of high-yield vector system, in which two GC-rich regions flanking the gene expression cassette have the promoter and enhancer continuously exposed to transcriptional machinery by preventing chromatin condensing. This revolutionary technology makes the highest yields available in all common mammalian cell protein production host cell lines, such as 293, CHO and NSO.
Antibody yields of up to 2.0- 5.5 grams/liter (80-120pg/cell/day) have been achieved utilizing this system in chemically defined, animal component-free medium. The selection strategy has been designed to identify cell lines that are highly productive and have the ability to grow well in suspension culture in a state-of-the-art, fed-batch fermentation process, in which cell density can reach 1.5×107/mL.
In addition, Creative Biolabs is also specialized in engineered bacterial construstruction and large scale fermentation. Its specific bacterial expression vector pCD-Bac is a temperature-inducible prokaryotic expression vector, which contains a PRPL promoter, cIts857 gene (temperature-sensitive CI repressor), multiple cloning sites and two strong transcription terminators. With genetic codons optimized into that bacterial preference, this vector enables high-yield protein expression of most proteins in the host bacterial strain (Escherichia coli DH5?) when their fermentation protocol is employed using NLF-22 fermentor.
Lear more about stable cell lines services of Creative Biolabs.
About Creative Biolabs
Creative Biolabs is an experienced biotech service provider from New York, USA. Since its establishment in 2005, Creative Biolabs has been focusing on the research and development of antibody production and antibody engineering. It has attracted a great number of customers from all over the world, including the USA, UK, Germany, Italy, Canada, etc. Learn more about Creative Biolabs.