Adenoviruses are major causes of febrile illness among children. They can infect the membranes of respiratory tract, intestines, eyes and urinary tract causing pharyngitis, pneumonia and gastroenteritis, bronchiolitis, hemorrhagic cystitis and conjunctivitis. There are 57 types of adenoviruses known to infect human beings, classified into seven categories from A to G on the basis of genome size and composition. Human Adenovirus A, B and C cause respiratory infection while HAdV B & D cause conjunctivitis. Children are more susceptible to adenovirus infections than adults and aging population. Late winter, early summer and spring are the most common time for this infection to occur. Adenoviruses are one of the major causes of respiratory infections among children and cause of diarrhea. As these infections are not specific, so they cannot be diagnosed on the basis of clinical symptoms only. A number of diagnostic methods used to confirm the presence of adenovirus infection include viral culture, polymerase chain reaction assays and antigen based assays. Others emerging technologies such as monoclonal antibodies, liposomes, flow cytometry, chromatography and gel microdroplets are of vital use in diagnosis of adenovirus infection.