Portable chromatography systems are person-portable; they are the instruments that are used outside of the lab. The portable chromatography system is moved with the help of the vehicle and they require external power. Several chromatography instruments work on the similar principle which is the partition of the sample mixture into mobile phase and stationary phase. In a large number of pharmaceutical and biotechnological organizations, the chromatography techniques are used throughout the year. The chromatography instruments have the ability to analyze, separate, and purify the molecules in an effective and efficient manner, thus has extensively helped the researchers. The various application areas where the portable chromatography system is used include pharmaceutical, food production, biotechnology, diagnostics, drug discovery, genetic engineering, water analysis, etc.