A blotting technique in molecular biology is defined as transfer of electrophoresed samples of DNA, RNA and proteins onto a suitable carrier such as nylon or nitrocellulose membrane and polyvinylidene difluoride (PVDF) membrane and subsequent fragment detection by probe hybridization. Southern blotting is a molecular biology technique used to detect specific DNA sequence in DNA samples. The technique is named after its inventor Edward Southern. Southern blotting technique combines transfer of electrophoresis separated DNA fragments to a filter membrane (nitrocellulose or nylon) and subsequent fragment detection by probe hybridization. Similar techniques involving separation of macromolecules and probing for certain sequences or motifs have now been developed to study RNA and protein named as northern blotting and western blotting techniques respectively. Southern blotting techniques is used in many areas such as in gene mapping, forensic and diagnostics studies. In regards to disease diagnosis the technique is used in diagnosis of congenital inherited disease such as congenital adrenal hyperplasia (CAH). In Southern blotting, DNA fragments that have been separated by gel electrophoresis from the intact gel is transferred onto a piece of nitrocellulose or nylon membrane. The membrane is then exposed to a hybridization probe—a single DNA fragment with a specific sequence whose presence in the target DNA (or RNA) is to be determined. The probe DNA is labeled so that it can be detected by radiography, fluorescence, or chemiluminescence. The demand for global southern blotting market is rising tremendously in medical, forensic, agricultural fields and drug discovery. In medical field, southern blotting technique is used for prognosis of cancer and in prenatal diagnosis of genetic diseases. Southern blotting technique also finds its application in diagnosis of HIV-1. In forensic science, southern blotting technique is used in sex determination and paternity testing. The global southern blotting market is expected to rise during the forecast period due to increasing number of healthcare disorders affecting the large pool of population coupled with technological advancement and increasing adoption of southern blotting technique in various biotechnology and pharmaceutical industries.